[Principle]
This reagent was prepared based on the principle of colloidal gold immunochromatography (GICA). After adding the sample to the sample well, it moves along the chromatographic membrane with the colloidal gold label. Red, if there is no cat calicivirus antigen in the sample, no color reaction will occur. This reagent uses cat calicivirus specific monoclonal antibodies and uses a sandwich method to determine the cat calicivirus specific antigen, which has high sensitivity and specificity.
[Kit composition]
No. | Name | 20T / box |
1. | test cards (including straws) | 20 |
2. | Sample dilution | 20bottles |
3. | Cotton swab | 20 sticks |
4. | instruction manual | 1 |
[Storage and expiry date] Store in a cool and dry place (2-30 ° C), valid for 24 months.
[Sample preparation]
After soaking the cotton swab with normal saline, swab the feces, rectum, vomit, and saliva. Immediately insert the cotton swab into the sample tube containing the diluent. Stir the cotton swab thoroughly until all the samples on the cotton swab are dissolved in the diluent. Place the cotton swab in the tube. The wall is squeezed out and discarded, and the sample solution is left to stand.
Note:
Excessive stool samples may lead to erroneous results (such as the occurrence of black bands, etc.), and can be diluted with the sample dilution solution before testing.
【experiment method】
1. Tear off the aluminum foil packaging bag of the test card, remove the test card, and place it on a flat, clean surface.
2. Pipette the prepared clear sample supernatant with a matching pipette, and slowly add 2-3 drops (about 60ul) into the sample well vertically and slowly.
3. Leave at room temperature for 5-10 minutes to judge the results. Results longer than 30 minutes are invalid.
